This study covers preparation of extracts and optimization of analytical parameters for injection, separation, and detection in Multi-residue Pesticides in Rice. The determination of pesticides in fruits and vegetables has been simplified by a QuEChERS extraction method. The Thermo Scientific ITQ 700 GC-ion trap MS showed excellent accuracy at low concentrations of 33 pesticide residues analyzed in rice. Using the instrument’s MSn functionality allows the user to identify, confirm, and quantify in one analytical run.